I started my day by getting ready to fill some new 96 well dishes. First I got the four drugs for the four dishes which were FdUMP[10], and Hairpins 1, 2, and 3. Then I placed those three drugs in a tray and looked for a 100 microleter multipipetter and a 100 microleter pipetter used to place the drug in the dishes. After that I checked to see if the pipetters were in good condition by pumping water through them. Since they looked okay I started the procedure. The first drug used was FdUMP[10], I vortexed the tube to bring all of the dug to the bottom and then used the pipetter to remove 100 microleters of the drug in each of the three wells under the four containing only medium. Then I used the multipipetter set to 100 microleters while using only three pipettes. I pumped the drug throughout the three wells all at once, ten times and moved on to the next three until I reached the last column. I followed the same process thoughout the next three trays using Hairpins 1, 2, and 3. All of the dishes contained 2,000 cells in each well except the outside wells which contained PBS and four of those wells on the bottom right contained only medium.

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