Today I checked on my cells to see if they had become confluent. Then I made and appoitment for the hood today at 12:00 PM, tomarrow at 9:00 AM and for friday. Then I entered in the information received from the data sheet I had gotten on thursday last week into the computer. Later I worked on my powerpoint presentation and then split my cells and placed them into new plates. Next I mixed the drugs for my experiment tomarrow in filling 96 well dishes. First I put the tubes of FdUMP[10], and Hairpins 1, 2, and 3 in the centrifuge to bring all of the drug to the bottom. Then I set a pipetter to 186 microleters and removed that amout from each of the four tubes and put it into four empty tubes. Next I added 93 microleters of PBS and 31 microleters of zinc chloride to each of the tubes while changing the tip every time. Then I placed the tubes in the centrifuge to bring all the drug to the bottom. Then I placed the tubes in the drybath and waited till the temperature reached 75 degrees to time it for 5 minutes. After the five minutes I removed the tubes from the drybath and left at room temperature for an hour. When the hour passed I put the tubes in a tray and placed it in the refrigerator. I did, accidently leave the drug in the water bath some minutes over, so now I have watch and see if the extra time in the drybath may have effected the drug. Later I continued to put in more information into the computer and printed out the graphs I had made from the data.