Today I mostly focused on finishing up my powerpoint presentation and added my graphs. I had to make a few miner ajustments so that the graphs would be visble and changed the back grounds to white. Later I ran some arrands for my lab tech and made reservations for the dinner next thursday.

First thing this morning I worked on my powerpoint presentation and wrote out some notes to help me. Then at 10:00 this morning I went with the lab tech to do an experiment at Wake Forest University to check a Buffer for RNAP. After writing some notes I watched as the graphs progressed and showed how much RNAP was present. At 12:00 PM we went back to our labs and left for lunch.
After lunch we got ready to go to Wake Forest again. We noticed the graphs were still quite simular at different temperatures.

I didn't too much to do today. I made sure to remove the dishes from the dryer this morning and then worked on my powerpoint presentation. Later I worked on my graphs and compared them to my first group of graphs. Then I put autoclaved tape on the dishes I removed from dryer to get them ready to be autoclaved.

I started my day by first going to the Kuchera lab at 9:00 clock. The lab tech then taught me how to count cells on the Hemosemtometer. I had to count DU-145 and PC3 cells, for the DU-145 cells I counted 77 and for the PC3 cells I counted 221. Later I put in the new numbers from the data sheet printed on Friday. Next I redid the concentration numbers and converted them to molecular weight. Then I went to the meeting with Dr. Lambros.

On Fridays the CERTL studens always have to meet in the computer lab for a workshop. I worked on my blog and my powerpoint while I was there, then I had to leave to put dye on my 96 well dishes that morning. After I was done a returned to the computer lab and continued to work on my blog. After our 30 minute break we had to meet with Addriene. We each discussed why we applied for CERTL and then were told of the meeting we had with Dr. Lambros on Monday.
After our lunch break we had to go to our chosen labs for Lab Visitation Day. I had signed for two labs with Dr. Harp and Dr. Williamson. After I went to the labs I came back to my lab and ran some arrands.

I went directy to the lab today to check on my cells. Then I watched as my lab tech split some cells and taught me how to freeze cells. As she went through the process of freezing the cells, I wrote a protocol for freezing cells. Later I put in some data for my graphs and then burned them on a clean CD to transfer to another computer. Then I wrote my introduction for my presentation and let Dr. Gmeiner look over it.

I started my day by getting ready to fill some new 96 well dishes. First I got the four drugs for the four dishes which were FdUMP[10], and Hairpins 1, 2, and 3. Then I placed those three drugs in a tray and looked for a 100 microleter multipipetter and a 100 microleter pipetter used to place the drug in the dishes. After that I checked to see if the pipetters were in good condition by pumping water through them. Since they looked okay I started the procedure. The first drug used was FdUMP[10], I vortexed the tube to bring all of the dug to the bottom and then used the pipetter to remove 100 microleters of the drug in each of the three wells under the four containing only medium. Then I used the multipipetter set to 100 microleters while using only three pipettes. I pumped the drug throughout the three wells all at once, ten times and moved on to the next three until I reached the last column. I followed the same process thoughout the next three trays using Hairpins 1, 2, and 3. All of the dishes contained 2,000 cells in each well except the outside wells which contained PBS and four of those wells on the bottom right contained only medium.

Today I checked on my cells to see if they had become confluent. Then I made and appoitment for the hood today at 12:00 PM, tomarrow at 9:00 AM and for friday. Then I entered in the information received from the data sheet I had gotten on thursday last week into the computer. Later I worked on my powerpoint presentation and then split my cells and placed them into new plates. Next I mixed the drugs for my experiment tomarrow in filling 96 well dishes. First I put the tubes of FdUMP[10], and Hairpins 1, 2, and 3 in the centrifuge to bring all of the drug to the bottom. Then I set a pipetter to 186 microleters and removed that amout from each of the four tubes and put it into four empty tubes. Next I added 93 microleters of PBS and 31 microleters of zinc chloride to each of the tubes while changing the tip every time. Then I placed the tubes in the centrifuge to bring all the drug to the bottom. Then I placed the tubes in the drybath and waited till the temperature reached 75 degrees to time it for 5 minutes. After the five minutes I removed the tubes from the drybath and left at room temperature for an hour. When the hour passed I put the tubes in a tray and placed it in the refrigerator. I did, accidently leave the drug in the water bath some minutes over, so now I have watch and see if the extra time in the drybath may have effected the drug. Later I continued to put in more information into the computer and printed out the graphs I had made from the data.

I started my morning by going straight to the computer lab for the computer workshop. The lab tech gave us some time to ourselves to gather any data we could for our prodject and gave us some tips on creating a powerpoint. After we had the computer workshop we were given a 30 minute break before we had to report to the conference room for our next meeting. During the meeting we read two articles, one about how fish oil affects those with artificial attachements to their hearts and the other article giving the positive results of drinking more milk for those who had PMS. I ended my day by respliting my cells for the long weeked and then read some articles given to me.